In order to propagate and cultivate Sequioa sempervirena,taking 63 clones introduced from France as samples,the study on techniques and measures of tissue culture of Sequioa sempervirens was carried out from the aspects of sample preparing,explant differentiation inducting,bud propagating,and root generating.The results showed that the optimum medium for inducing bud differentiation was MS+6-BA1.5 mg·L-1+KT2.0 mg·L-1,root medium was 1/2MS+IBA1.0 mg·L-1+NAA2.0 mg·L-1.

采用温室盆栽的方法探讨了北美红杉1年生幼苗对不同N供应水平(0、0.05、0.11、0.16、0.21、0.32、0.42和0.63 g.L-1,0.21 g.L-1为对照)的生长反应,结果表明:(1)幼苗在完全供N水平(对照)时,苗高净增量为31.2cm;当供N水平降到一半(0.11 g.L-1)以下时(N3~N1),苗木的生长明显减缓,苗高净增量分别是对照的92.6%、83.0%和29.8%;供N水平增加2倍(0.63 g.L-1)时,苗高净增量为对照的70.8%。(2)当N供应水平降低(N4~N2)或缺N(N1)时,幼苗地下部分与地上部分比率(R/S)增加,缺N时为对照的2.1倍;净增生物量分配给根系的比例也明显增大,缺N时为56%。(3)在低N供应条件下,幼苗细根(D≤2 mm)的特定根长增加尤为明显,缺N时为14.9 m.g-1,是对照的169%;N供应过量时,细根的特定根长减小,在0.63 g.L-1供N水平(N8)时为6.0 m.g-1,约为对照的68%。